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KMID : 0364819640020010001
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Korean Journal of Microbiology
1964 Volume.2 No. 1 p.1 ~ p.11
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Studies on the phosphate metabolism in chlorella, with special reference to polyphosphate
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Abstract
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Yung Nok Lee (Dept. of Biology, Korea University) : Studies on the phosphate metabolism in Chlorella, with special reference to polyphosphate. Kor. J. Microbiol., Vol.2, No.1, p1-11 (1964). 1. Uniformly ³²P-labeled Chlorella cells which were irradiated with Cobalt-60 gamma-rays of about 70,000γ dose, were further grown in a standard "cold" medium ("hot" → "cold"), and some portions of the algae were taken out at the begining of, and at intervals during the culture, and subjected to analyze the contents of ³²P and total P in various fractions of the cell materials. Results obtained were compared with those of nonirradiated normal cells. 2. Amounts of phosphate in various fractions of the nonirradiated normal Chlorella cells were measured using uniformly ³²P-labeled cells. Analysis of the ³²P-labeled algal cells showed that the highest value in P-content was the fraction of RNA followed by those of lipid, polyphosphate "C" polyphosphate "B", DNA, nucleotidic labile phosphate compounds, polyphosphate "A" and protein. It was observed that content of total polyphosphates in a single Chlorella cell was almost equal to RNA-P content in the cell, and the amount of RNA-P was almost equal to ten times of DNA-P content. 3. When the ³²P-labeled algae which were irradiated with gamma-rays were grown in a normal "cold" medium, phosphate contents in the fraction of DNA, nucleotidic labile phosphate compounds and protein decreased markedly, while the contents of phosphate in the fractions of polyphosphate "C" and polyphosphate "B" increased in comparison with those of unirradiated normal cells. So, it was considered that the pretreatment of above mentioned dose of gamma-ray inhibited DNA and protein synthesis from polyphosphate in Chlorella cells. 4. Proceeding the culture of ³²P-labeled Chlorella in a "cold" standard medium, whose synthetic activity of DNA and protein from polyphosphate was disturbed by gamma-ray irradiation, the amounts of ³²P in the fraction of polyphosphate "C" increased, in contrast with those of polyphosphate "B" fraction. According to these experimental results, it was inferred that polyphosphate "B" could transform into polyphosphate "C" in normal growing Chlorella cells.
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